Owing to the difficulty of culturing clinical isolates, use of surrogate experiments are warranted20. A representative set of 10 sequences was selected and also sequenced using the Illumina MiSeq platform. b, Magnification of the region defined by the box around H69 in a. H69 is highlighted in yellow. J. Clin. He is a popular Indian Film Critic, a Motivational Speaker, and a renowned Leadership Consultant. The relationship of the divergent samples to those at earlier time points argues against superinfection. Sort. Source data. Notably, spike(N501Y)—which can increase the affinity for the ACE2 receptor6 and which is present in the UK B1.1.7 lineage7—was observed on day 55 at a frequency of 33%, but was eliminated by the sweep of the NSP2 and RdRp variant. The serum dilution required to inhibit 50% of virus infection (ID50) is shown, expressed as a fold change relative to the wild-type virus. März 2020 Britische Mediziner haben den zweiten HIV-Patient geheilt Der sogenannte "London-Patient" hat eine spezielle Stammzellentherapie bekommen (…) In brief, amplicons were end-repaired and had A-overhang added; these were then ligated with 15 mM of NEXTflex DNA Barcodes (Bio Scientific). Palmer, S. et al. ", Professor Ravindra Gupta, a biologist of Indian origin has also made it to the list. is an ace media professional with more than 20 years of experience in the field of Media and Advertising. Variant frequencies were validated using custom code as part of the AnCovMulti package (https://github.com/PollockLaboratory/AnCovMulti). Ravindra GUPTA | Cited by 3,238 | of University of London, London | Read 121 publications | Contact Ravindra GUPTA After clinical deterioration, remdesivir and a unit of convalescent plasma were administered on day 95, but the individual died on day 102 (Supplementary Note). Whole blood was diluted 1:5 in RPMI into 96-well F plates (Corning) and activated by single stimulation with phytohaemagglutinin (10 μg ml−1; Sigma-Aldrich), lipopolysaccharide (1 μg ml−1, List Biochemicals) or by costimulation with anti-CD3 (MEM57, Abcam, 200 ng ml−1, 1:1,000) and IL-2 (Immunotools, 1,430 U ml−1, 1:1,000) antibodies. These viral populations were then replaced by virus carrying spike(W64G) and spike(P330S), which both dominated at day 93. 2). Although the samples that we obtained on days 93 and 95 of infection are genetically distinct from the other samples, the remaining samples are consistent with arising from a consistent viral population. Red indicates significant fold changes. All samples were tested for presence of SARS-CoV-2 with a validated one-step RT–qPCR assay developed in conjunction with the Public Health England Clinical Microbiology22. University of Cambridge. D796 is located near the base of the spike protein, in a surface loop that is structurally disordered in the prefusion conformation and becomes part of a large disordered region in the post-fusion S2 trimer11 (Extended Data Fig. The elderly women were accorded a prominent space in the front row on each day of the protests. Finally, we detected no evidence of recombination, based on two independent methods (data not shown). Amplification reactions were all performed on a Rotorgene PCR instrument. Hier sollte eine Beschreibung angezeigt werden, diese Seite lässt dies jedoch nicht zu. 9 Location of spike mutations ΔH69/Y70 and D796H. In the RBD-down spike structures11,12, D796 forms contacts with residues in the neighbouring protomer, including the glycosylated residue N709 (Extended Data Fig. Illingworth, C. J. SAMFIRE: multi-locus variant calling for time-resolved sequence data. In our case, we detected environmental contamination while in a single-occupancy room and the patient was moved to a negative-pressure high air-change infectious-disease isolation room. eLife 9, e56915 (2020). To obtain We also used an HIV-1(p24) antibody to monitor the levels of lentiviral particle production (Fig. On days 86 and 89, viruses obtained from upper respiratory tract samples were characterized by the spike(Y200H, T240I) double mutant, with the mutation–deletion pair (spike(D796H, ΔH69/ΔV70)) observed on day 82 having decreased to frequencies of 10% or less (Figs. Cryo-EM structures of SARS-CoV-2 spike without and with ACE2 reveal a pH-dependent switch to mediate endosomal positioning of receptor-binding domains. After 48 h incubation in a 5% CO2 environment at 37 °C, luminescence was measured using a Bright-Glo Luciferase assay system (Promega) and neutralization was calculated relative to virus-only controls. Proc. Internal distances between samples in the proposed main clade are shown in black, distances between samples in the main clade and samples collected on days 93 and 95 are shown in red, and internal distances between samples collected on days 93 and 95 are shown in green. Google Scholar. b, Single-genome sequencing data from respiratory samples at the indicated days. PubMed The observation of potential selection for specific variants coinciding with the presence of antibodies from convalescent plasma is supported by the experimental finding of the twofold reduced susceptibility of these viruses to convalescent plasma containing polyclonal antibodies. Extended Data Fig. The filtrate was centrifuged at 15,000 rpm for 120 min to pellet virions. Cited by . https://www.cdc.gov/coronavirus/2019-ncov/hcp/disposition-hospitalized-patients.html (2020). & McCauley, J. GISAID: global initiative on sharing all influenza data — from vision to reality. He also revealed that his surgery was done and dusted. Bioinformatics 34, 3094–3100 (2018). 2019 Mar 5. doi: 10.1038/s41586-019-1027-4. Point of care nucleic acid testing for SARS-CoV-2 in hospitalized patients: a clinical validation trial and implementation study. Consensus sequences from short-read deep-sequencing Illumina data revealed dynamic population changes after day 65, as shown in a highlighter plot (Extended Data Fig. Share to Twitter Share to Facebook Share to Pinterest. Nucleic acid extraction was done from 500 μl of sample with a dilution of MS2 bacteriophage to act as an internal control, using the easyMAG platform (Biomerieux) according to the manufacturers’ instructions. Monday, 18 April 2016. Tag Archiv: Ravindra Gupta 11. Cytokines were measured by multiplexed particle-based flow cytometry on a Luminex analyser (Bio-Plex, Bio-Rad) using an R&D Systems custom kit (R&D Systems). Tackling COVID-19: Professor Ravi Gupta. Cambridge physician and microbiologist Professor Ravindra Kumar Gupta is one of six persons of Indian-origin on the latest Time 100 Most Influential People 2020 list.. Preprint at https://doi.org/10.1101/2020.11.18.20230599 (2020). Ravindra Jadeja injury update: Southpaw completes successful surgery. a, Variants detected in patient X1 on days 1–82 after the first positive RT–qPCR test for SARS-CoV-2. Natl Acad. Virus Evol. 217, e20201181 (2020). a, At baseline, all six spike variants (Illumina sequencing) except for spike(ΔH69/ΔV70) were absent (less than 1% and fewer than 20 reads). EMBnet J. Natl Acad. Source data are provided with this paper. Extended Data Fig. Indian names such as Narendra Modi, Ayushmann Khurrana, Sundar Pichai, Ravindra Gupta, and Bilkis Bano have featured in TIME magazine's list of 100 influential people of 2020. Ravindra K. Gupta. Lumby, C. K., Zhao, L., Breuer, J. Sort by citations Sort by year Sort by title. Assistant Professor, King Saud University. This is a report of a single case and therefore limited conclusions can be drawn about generalizability. CAS Environmental sampling showed evidence of virus on surfaces such as the telephone and call bell. J. Med. USA 105, 7552–7557 (2008). 8 Neutralizing activity of a panel of monoclonal antibodies against SARS-CoV-2 spike-pseudotyped viruses. Nat. L.E.M. Struct. In his piece about Gupta for Time, Castillejo said, "Through the years, our partnership has developed and strengthened as Gupta has shared his knowledge and his … Although we await further data, in cases in which clinical trial enrolment is not possible, convalescent plasma administered for clinical need in immunosuppressed individuals should ideally only be considered as part of observational studies, undertaken preferably in single-occupancy rooms with enhanced infection-control precautions, including environmental sampling of SARS-CoV-2 and real-time sequencing.